Preparation Instructions:

To prepare LB Miller Broth, mix 25 g of the medium in 1 L of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Then, distribute and autoclave at 121.0⁰C for 15 minutes.

Store at room temperature, tightly sealed, and protect from moisture and light.

Other Details:

Physical Specifications:

CAS#:N/A
pH (@ 25°C):7.0 ± 0.2
NOTE: Grams per liter may be adjusted to obtain desired performance.

Molecular Biology Specifications:

Escherichia coli ATCC 23724: Growth
Escherichia coli ATCC 33694: Growth
Escherichia coli ATCC 33849: Growth
Escherichia coli ATCC 39403: Growth
Escherichia coli ATCC 47014: Growth
Escherichia coli ATCC 53868: Growth

LB Miller Broth Specification Sheet

Certificate of Analysis Link:

LB Miller Broth CofA - Lot#19A3011

LB Miller Broth CofA-_Lot#19F2002

LB Miller Broth CofA- Lot#19G5001

LB Miller Broth CofA- Lot#19H5004

LB Miller Broth CofA- Lot#19I5001

LB Miller Broth CofA- Lot#20A2002

LB Miller Broth CofA_-_Lot#20E3013

LB Miller Broth CofA_-_Lot#20F1005

LB Miller Broth CofA -_Lot#20H1003

LB Miller Broth CofA -_Lot#20H4015

LB Miller Broth CofA_-_Lot#21C2002

LB Miller Broth CofA_-_Lot#21G2004

LB Miller Broth CofA - Lot#21J2003

LB Miller Broth CofA -Lot#22K3003

LB Miller Broth CofA - Lot#23B2001

LB Miller Broth CofA - Lot#23E2014

LB Miller Broth CofA - Lot#23H3023

LB Miller Broth CofA - Lot#23L3001

LB Miller Broth CofA - Lot#24A4012

LB Miller Broth CofA - Lot#23M2003

LB Miller Broth CofA - Lot#24C2001

LB Miller Broth CofA - Lot#24C3004

SDS Link:

LB Miller Broth - SDS

Formula per Liter:

● Casein Digest Peptone 10.0 gm
● Sodium Chloride 10.0 gm
● Yeast Extract 5.0 gm

Preparation:

● Mix 25 gm of the medium in 1 L of purified water until evenly dispersed.
● Heat with repeated stirring, and boil for one minute to dissolve completely.
● Distribute and autoclave at 121.0°C for 15 minutes.

Recommended Use:

IBI’s Luria Broth (LB Miller) is used in molecular genetic studies, as well as maintaining and propagating E.coli in molecular and microbiology procedures. The inclusion of casein peptone and yeast extract supply essential growth factors; such as nitrogen, sulfur, minerals, and vitamins. Sodium chloride provides essential electrolytes.

Storage:

Store at room temperature. Keep tightly sealed. Protect from moisture and light.

Warning:

Irritant. Irritating to the eyes, respiratory system, and skin. Wear suitable protective clothing, gloves, and eye/face protection. See Material Safety Data Sheet for additional information.